Do you want to remove the Spaces icon in your Twitter navigation bar? You'll need to subscribe to Twitter Blue. Twitter has finally brought this option to Android users. (iOS Twitter Blue subscribers got first dibs on the feature.) You can now tweak your navigation bar so it'll only show the features you use frequently.
This won't entirely get rid of Spaces in your timeline. In fact, Twitter is doubling down on bringing the audio feature to your attention. You'll see more information on active Spaces in a banner at the top of your timeline, and neither paid nor free users can get rid of this banner.